The Biochemistry of Tardigrades
The Kolling Lab’s research is focused on elucidating the biochemical mechanisms by which tardigrades survive extreme environmental stress.
To do this, researchers conduct a variety of biochemical assays and employ chemical analytical methods to evaluate the underpinnings of cryptobiosis.
Current Projects:
Physiological Characterization
In order to understand cryptobiosis (the process by which tardigrades withstand environmental stress) we have to characterize the different physiological states formed in response to various stressors. This includes:
- Tun formation assays
- Microscopic techniques (laser scanning confocal fluorescence microscopy; scanning electron microscopy)
Free Radical Quantification
Previous studies by the Kolling Lab in conjunction with researchers at UNC Chapel Hill have found that Reactive Oxygen Species (ROS) are a key component in cryptobiosis induction. We use Electron Paramagnetic Resonance (EPR) and antioxidant studies to probe these relationships.
Metabolic Studies
We are also interested in the tardigrade’s unique possession of the Alternative Oxidase Pathway (AOX) and the role it and other Electron Transport Chain Complexes play in metabolism and tun formation.
Bacteria-Host Interaction Studies
Like humans, tardigrades also interact with their internal and external microbiome. We are currently seeking to understand the tardigrade gut microbiome, as well as the defenses tardigrades employ to defend themselves against pathogens in their environment.
The Kolling Lab 